vtn antibody  (Proteintech)

Journal: International journal of molecular sciences

Article Title: Using Cancer-Associated Fibroblasts as a Shear-Wave Elastography Imaging Biomarker to Predict Anti-PD-1 Efficacy of Triple-Negative Breast Cancer.

doi: 10.3390/ijms26083525

Figure Lengend Snippet: Figure 1. Wound-healing CAFs are correlated with immunosuppression and tumor growth rate. (A) Gene ontology biological process (GOBP) enrichment analysis was performed based on CAF gene expression in different CAF subtypes and the focused pathways and subtypes are marked in red. (B) Dot plot showing different types of fibroblast growth factor (FGF) expression in various subtypes of CAFs. (C) Interleukin-6 (IL-6) and C-X-C motif chemokine ligand 12 (CXCL12) expres- sion in each subtype of CAFs. (D) Several proteins that are only highly expressed on WH CAFs. (E) Immunofluorescence staining of vitronectin (VTN) (red) distribution in tumors, scale bar: 100 µm. (F) VTN immunofluorescence quantification results of selected tumors (n = 5 independent samples). (G) Comparison of tumor volume from high- and low-proportion groups on the last day (n = 5 independent samples). (H) Selected tumor volume curves from high- and low-proportion groups over 14 days (n = 5 independent samples). (I) Mean selected tumor volume curve compari- son from high- and low-proportion groups over 14 days (n = 5 independent samples). (J) Sirius red staining in high and low groups, scale bar: 1.25 mm (low magnification), 100 µm (high magnification). (K,M) Western blot analysis of collagen I and IL-6 protein in high- and low-proportion groups. (L) The immunofluorescence co-staining image of CD8 (green) and VTN (red), scale bar: 100 µm (1: Extracellular Matrix CAFs; 2: Matrix CAFs 1; 3: Development CAFs; 4: Antigen presentation CAFs 1; 5: Antigen presentation CAFs 2; 6: Wound healing CAFs; 7: Vascular CAFs; 8: Inflammatory CAFs; 9: Matrix CAFs 2). Data expressed as mean ± S.E.M; statistical comparisons were performed using Student’s t test.

Article Snippet: The primary antibodies used for IF staining include VTN antibody (Proteintech, Wuhan, China, 66398-1-lg), FGFR2 antibody (Proteintech, Wuhan, China, 13042-1-AP), CD8a antibody (Proteintech, Wuhan, China, 29896-1-AP), and SHMT1 antibody (Proteintech, Wuhan, China, 67963-1-Ig).

Techniques: Gene Expression, Expressing, Immunofluorescence, Staining, Comparison, Western Blot, Immunopeptidomics

Journal: International journal of molecular sciences

Article Title: Using Cancer-Associated Fibroblasts as a Shear-Wave Elastography Imaging Biomarker to Predict Anti-PD-1 Efficacy of Triple-Negative Breast Cancer.

doi: 10.3390/ijms26083525

Figure Lengend Snippet: Figure 3. Deep learning model to evaluate WH CAF level. (A) Representative SWE image of mice; each image is composed of a pseudo-color image and grayscale image. (B) Quantification results of VTN immunofluorescence staining. The solid red line represents the average (n = 105 independent samples). (C) Representative immunofluorescence staining of VTN (red) from high and low level, scale bar: 100 µm. (D) Mean stiffness value in high- and low-level groups (WH high-level group: n = 62, WH low-level group: n = 43). (E) The framework of our proposed deep learning model. (F) Performance of the deep learning model to predict the WH CAFs level. ROC: receiver operating characteristic curve; AUC, area under the curves. (G) Confusion matrices in two classification cate- gories. The confusion matrices showed the pairwise comparison. Data expressed as mean ± S.E.M; statistical comparisons were performed using Student’s t test.

Article Snippet: The primary antibodies used for IF staining include VTN antibody (Proteintech, Wuhan, China, 66398-1-lg), FGFR2 antibody (Proteintech, Wuhan, China, 13042-1-AP), CD8a antibody (Proteintech, Wuhan, China, 29896-1-AP), and SHMT1 antibody (Proteintech, Wuhan, China, 67963-1-Ig).

Techniques: Immunofluorescence, Staining, Comparison

Journal: International journal of molecular sciences

Article Title: Using Cancer-Associated Fibroblasts as a Shear-Wave Elastography Imaging Biomarker to Predict Anti-PD-1 Efficacy of Triple-Negative Breast Cancer.

doi: 10.3390/ijms26083525

Figure Lengend Snippet: Figure 4. FGFR inhibitor potentiates immune checkpoint inhibitors (ICIs) in WH CAF high-level tumors. (A) Schematic of the experimental protocol. SWE: shear wave elastography. (B) Representa- tive SWE images of tumors in high-level and low-level groups at the end of volume measurement. (C) Mean stiffness value of tumors in high-level and low-level groups (WH high-level group: n = 27, WH low-level group: n = 14). (D) Tumor volume curves in different groups during the 15-day monitoring period (Group: WH CAFs high level, WH CAFs high level with Anti-PD-1, WH CAFs high level with Anti-PD-1+Erdafitinib: n = 9; group: WH CAFs low level, WH CAFs low level with Anti-PD-1: n = 5; group: WH CAFs low level with Anti-PD-1+Erdafitinib: n = 4). (E) Comparison of immunohistochemical results of CD8 and CD3 in high- and low-level groups before and after Anti- PD-1+Erdafitinib treatment, scale: 100 µm. (F) Immunofluorescence staining and quantization results of FGFR2 and VTN proteins in WH CAFs high-level group before and after Anti-PD-1+Erdafitinib treatment, scale bar: 2000 µm (low magnification), 100 µm (high magnification). Data expressed as Mean ± S.E.M; statistical comparisons were performed using Student’s t test.

Article Snippet: The primary antibodies used for IF staining include VTN antibody (Proteintech, Wuhan, China, 66398-1-lg), FGFR2 antibody (Proteintech, Wuhan, China, 13042-1-AP), CD8a antibody (Proteintech, Wuhan, China, 29896-1-AP), and SHMT1 antibody (Proteintech, Wuhan, China, 67963-1-Ig).

Techniques: Shear, Comparison, Immunohistochemical staining, Immunofluorescence, Staining

Journal: NPJ Biofilms and Microbiomes

Article Title: Clostridium difficile -derived membrane vesicles promote fetal growth restriction via inhibiting trophoblast motility through PPARγ/RXRα/ANGPTL4 axis

doi: 10.1038/s41522-024-00630-5

Figure Lengend Snippet: A The protein expression levels of β-actin, FN1 and VTN were detected by western blotting. B The mRNA expression levels of FN1 and VTN was detected by qRT-PCR. C Adhesion ability of the HTR-8/SVneo cells treated with C. difficile MVs 5 μg/ml was detected by adhesion experiment. D Migration ability of the HTR-8/SVneo cells treated with C. difficile MVs 5 μg/ml for 24 h was detected by wound-healing assay. E Transwell assay was used to analyze the migration and invasion capabilities of HTR-8/SVneo cells treated with C. difficile MVs 5 μg/ml for 24 h. Data are represented as mean ± SD including at least three replicates. Data was analyzed using t -test. * P < 0.05; ** P < 0.01; *** P < 0.001.

Article Snippet: Membranes were incubated with the following antibodies: FN1 (1:2000, 15613-1-AP, Proteintech), VTN (1:10000, 66398-1-Ig, Proteintech), PPARγ (1:1000, 60127-1-Ig, Proteintech), and β-actin (1:10000, 66009-1-Ig, Proteintech).

Techniques: Expressing, Western Blot, Quantitative RT-PCR, Migration, Wound Healing Assay, Transwell Assay

Journal: NPJ Biofilms and Microbiomes

Article Title: Clostridium difficile -derived membrane vesicles promote fetal growth restriction via inhibiting trophoblast motility through PPARγ/RXRα/ANGPTL4 axis

doi: 10.1038/s41522-024-00630-5

Figure Lengend Snippet: A The inhibitory effect of C. difficile MVs on HTR-8/SVneo cell adhesion was mitigated by T0070907 and PPARγ shRNA. B Down-regulation of FN and VTN by C. difficile MVs was eliminated by T0070907 and PPARγ shRNA in HTR-8/SVneo cells. C , D The inhibition of C. difficile MVs on the migration and invasion of HTR-8/SVneo cells was alleviated by T0070907 and PPARγ shRNA. Data are represented as mean ± SD including at least three replicates. Data was analyzed using ANOVA with Tukey’s post hoc test. * P < 0.05; ** P < 0.01; *** P < 0.001.

Article Snippet: Membranes were incubated with the following antibodies: FN1 (1:2000, 15613-1-AP, Proteintech), VTN (1:10000, 66398-1-Ig, Proteintech), PPARγ (1:1000, 60127-1-Ig, Proteintech), and β-actin (1:10000, 66009-1-Ig, Proteintech).

Techniques: shRNA, Inhibition, Migration

Journal: NPJ Biofilms and Microbiomes

Article Title: Clostridium difficile -derived membrane vesicles promote fetal growth restriction via inhibiting trophoblast motility through PPARγ/RXRα/ANGPTL4 axis

doi: 10.1038/s41522-024-00630-5

Figure Lengend Snippet: A Protein expression levels of PPARγ, ANGPTL4 and RXRα were elevated in the FGR placenta, and the levels of VTN and FN1 were decreased. B The mRNA expression level of PPARγ was significantly increased in the placenta of FGR. Data are represented as mean ± SD including at least three replicates. Data was analyzed using t -test. * P < 0.05; ** P < 0.01; *** P < 0.001. C – G Correlations between the relative mRNA expression level of PPARγ and patient clinical parameters. Spearman’s correlation test was used for statistical analysis. H The schematic diagram of C. difficile and its MVs involvement in FGR.

Article Snippet: Membranes were incubated with the following antibodies: FN1 (1:2000, 15613-1-AP, Proteintech), VTN (1:10000, 66398-1-Ig, Proteintech), PPARγ (1:1000, 60127-1-Ig, Proteintech), and β-actin (1:10000, 66009-1-Ig, Proteintech).

Techniques: Expressing

Journal: NPJ Biofilms and Microbiomes

Article Title: Clostridium difficile -derived membrane vesicles promote fetal growth restriction via inhibiting trophoblast motility through PPARγ/RXRα/ANGPTL4 axis

doi: 10.1038/s41522-024-00630-5

Figure Lengend Snippet: Primers used in qRT-PCR

Article Snippet: Membranes were incubated with the following antibodies: FN1 (1:2000, 15613-1-AP, Proteintech), VTN (1:10000, 66398-1-Ig, Proteintech), PPARγ (1:1000, 60127-1-Ig, Proteintech), and β-actin (1:10000, 66009-1-Ig, Proteintech).

Techniques: Sequencing